如何限制应用程序仅从已知的USB闪存驱动器运行?

您可以检查卷序列号,这将捕获到临时复制到新格式化的卷,但它不会检测到完全字节精确的卷拷贝。
To protect software by accessing harddisk serial no
Any faster method to get Volume Serial number?

您可以检查运行程序的驱动器类型:

string path = Process.GetCurrentProcess().MainModule.FileName;
FileInfo fileInfo = new FileInfo(path);
string driveRoot = fileInfo.Directory.Root.Name;
DriveInfo driveInfo = new DriveInfo(driveRoot);
if (driveInfo.DriveType != DriveType.Removable)
{
    MessageBox.Show("Must run from removable drive");
    Application.Exit();
}